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| Brassesco,María Sol; Montaldi,Ana Paula; Gras,Diana Ester; Queiroz,Rosane Gomes de Paula; Martinez-Rossi,Nilce Maria; Tone,Luiz Gonzaga; Sakamoto-Hojo,Elza Tiemi. |
| Chromosomal translocations are characteristic of hematopoietic neoplasias and can lead to unregulated oncogene expression or the fusion of genes to yield novel functions. In recent years, different lymphoma/leukemia-associated rearrangements have been detected in healthy individuals. In this study, we used inverse PCR to screen peripheral lymphocytes from 100 healthy individuals for the presence of MLL (Mixed Lineage Leukemia) translocations. Forty-nine percent of the probands showed MLL rearrangements. Sequence analysis showed that these rearrangements were specific for MLL translocations that corresponded to t(4;11)(q21;q23) (66%) and t(9;11) (20%). However, RT-PCR failed to detect any expression of t(4;11)(q21;q23) in our population. We suggest that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genomic instability; Lymphocytes; MLL rearrangements. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200005 |
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| Brassesco,María Sol; Montaldi,Ana Paula; Sakamoto-Hojo,Elza Tiemi. |
| Topoisomerase II inhibitors are effective chemotherapeutic agents in the treatment of cancer, in spite of being associated with the development of secondary leukemia. Our purpose was to determine the effects of etoposide on different genomic regions, aiming at discovering whether there are preferential sites which can be targeted by this drug in peripheral lymphocytes from healthy individuals. The in vitro treatment with low doses of etoposide (0.25, 0.5, and 1 µg/mL, in 1 hour-pulse or continuous-48 h treatment) induced a significant increase in chromosomal aberrations, detected by conventional staining and FISH with specific probes for chromosomes 8 and 11, compared with untreated controls (p < 0.05). Additionally, the frequencies of alterations at... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Etoposide; FISH; MLL translocations. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100022 |
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